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Getting Started

Welcome Installing Bioconductor Command line tools (Docker) Getting the data

Quality Control

Overview Depth of Sequencing Coverage Cross Sample Contamination Tumor-in-normal Tumor Purity

Somatic Mutation Calling

Overview MuTect Strelka MuTect2

Allelic Copy Number Calling

Overview FACETS GATK ACNV

Mutational Signatures

Overview SomaticSignatures (NMF) deconstructSigs (NNLS) SigMa (Likelihood) scarHRD (Homologous Recombination Deficiency) MSIsensor (Microsatellite Instability)

Mutational Significance Analysis

Overview MutSigCV OncodriveFML MutPanning Brown's Method (for combining p-values)

Tumor Phylogenetics

Overview Calculate Mutation Cancer Cell Fractions (CCFs) PhylogicNDT PyClone

Gene Set Analysis

Overview GSEA ssGSEA Pathway Overrepresentation

Differential Expression

Overview edgeR DESeq2 Independent Hypothesis Weighting (IHW)

PyClone

Generating the mutation file

Running PyClone

https://www.anduril.org/anduril/bundles/all/doc/index.html?c=sequencing.PyClone

PyClone run_analysis_pipeline --in_files ${sep =" " mutation_files} \
--tumour_contents ${sep =" " purities} \
--samples ${sep =" " sample_names} \
--density pyclone_beta_binomial \
--num_iters 10000 \
--prior major_copy_number \
--burnin 1000 \
--thin 1 \
--seed 42 \
--mesh_size 101 \
--min_cluster_size 2 \
--working_dir ~/Downloads

Markov Chain Monte Carlo (MCMC)


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